Journal: Acta biomaterialia
Article Title: Decellularized Neonatal Cardiac Extracellular Matrix Prevents Widespread Ventricular Remodeling in Adult Mammals after Myocardial Infarction
doi: 10.1016/j.actbio.2019.01.062
Figure Lengend Snippet: (A) In vitro HUVEC migration assays show significantly higher migration in response to nmECM than saline or amECM. (B) In oxygen-deprived (2.5% O2) and nutrient-deprived culture conditions, nmECM significantly preserves cell activity relative to amECM or 2% FBS control. (C) Representative images of CD31+ endothelial cells and surrounding αSMA+ smooth muscle cells reveal vasculature in uninjured hearts and in response to each treatment after MI (scale bar = 50μm). Quantification of CD31+ (D) and αSMA+ (E) cells reveal increased density of vascular cells in response to both amECM and nmECM, although nmECM produces significantly more than amECM-treated mice. The density of CD31+ blood vessels (F) and blood vessels surrounded by αSMA-expressing cells (G) showed a similar trend. (n = 4 per treatment group in all panels; *p < 0.05, **p < 0.01 relative to saline; #p < 0.05 relative to amECM).
Article Snippet: Measurement of Endothelial Cell Activity in vitro Human umbilical vein endothelial cells (HUVEC, ATCC, Manassas, VA) were cultured in EGM-2 media (Lonza, Basel, Switzerland) and grown on standard tissue culture polystyrene dishes for all experiments.
Techniques: In Vitro, Migration, Saline, Activity Assay, Control, Expressing